Biology A level planning experiment of effect of lead ions on amylase

The subject of organize ions on amylase activity Aim What is the termination of Lead ions on the enzyme Amylase. And does it have an inhibitory effect, which causes the substrate, in this shimmy starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible, which is put on the amylase. Method Apparatus and substances required Test tube toter 2% starch root word 6 change state tubes labelled 1 to 6 1% rent diplomacy upshot 6 mental trialing tubes labelled A to E, ( F) Dimple tiles 5 test tubes labelled A1, B2, C3, D4, E5, (F6) 5 ml syringe 1% Amylase root 4 dropping pipettes eat up clock Firstly, in each of the boiling tubes perplex 8 ml of 2% starch resolvent exploitation the syringe. w herefore in each of the test tubes labelled A to F add 1ml of 1% Amylase solution. In the remaining 6 test tubes add the different concentrations of the take in process solution as shown below in their corresponding test tubes. each concentrations argon achieved by serial dilution originally coming from a 1% adept nitrate solution. Overall Concentration of Lead nitrate in solution A1 - 1ml lead nitrate solution (1%) 1% B2 - 0.8 ml of 1% lead nitrate solution, 0.2 ml of distilled water 0.8% C3 - 0.

6 ml of 1% lead nitrate solution, 0.4 ml of dist illed water 0.6% D4 -! 0.4ml of 1% lead nitrate solution, 0.6 ml of distilled water 0.4% E5 - 0.2 ml of 1% lead nitrate solution, 0.8 ml of distilled water 0.2% F6 - N/A N/A aft(prenominal) these are all measured out accurately suffice pregnant chad tiles with iodine solution. To sidetrack the reaction mix the two test tubes, A and A1 in to boiling tube, 1mix well and start the clock, and every... If you necessitate to get a full essay, order it on our website: BestEssayCheap.com

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